Welcome!
The purpose of the Vanderbilt Mass Spectrometry Research Center (MSRC) Core is to provide state of the art instrumentation and expertise for investigators needing mass spectrometry-based services. Because of different instrumentation and sample preparation requirements, the offered services are subdivided into three Cores: Small Molecule and Metabolomics, Proteomics, and Tissue Imaging. For most small-molecule projects, the user will receive training and assistance in developing their assay and then sign up for time on one of the open-access instrument platforms. For Metabolomics, Proteomics and Tissue Imaging, projects are managed collaboratively with one of the Associate Directors who will assist in planning the appropriate sample preparation, data acquisition, and data analysis strategies. Please read the following descriptions of Core services and choose the most appropriate Core to handle your project.
Small Molecule / Metabolomics Core
The MS/Metabolomics Core offers use access to MALDI-TOF, LC-MS, GC-MS, and ICP-MS instruments. Faculty, trainees, and staff of Vanderbilt investigators can be trained on the available instruments. Core personnel are also available to run samples for those investigators with infrequent needs or with sophisticated methods. MS Core personnel train new users, maintain the equipment, tune and calibrate the instruments, assist users with setting up and operating the instruments, and assist with data analysis and interpretation. The Core Associate Director, Dr. Wade Calcutt, is familiar with GLP protocols and FDA guidelines for bioanalytical methods development for sample processing, analysis and data reporting and is available to assist investigators for such projects.
One GC-MS single quadrupole and ten tandem LC-MS instruments are available for analysis of small molecules for drug metabolism and PK/PD studies and for quantitation of endogenous biomolecules and metabolites. Two Orbitraps and one Synapt Q-TOF instrument are available for high resolution, accurate mass determinations of molecular structures. Most mass spectrometers in the Core can be interfaced with reverse-phase, normal-phase, or nanoflow LC chromatography systems. One MALDI-TOF instrument is available. A Thermo Element 2 high resolution sector ICP-MS instrument and an Agilent 7700 single quadrupole ICP-MS instrument are available absolute quantitation of elements in liquid digested samples. The Thermo Element is equipped with a laser ablation system for trace element tissue imaging. A recently acquired Thermo Q Exactive HF instrument is dedicated to targeted and untargeted metabolomic and lipidomics studies.
M. Wade Calcutt, Research Assistant Professor
Associate Director of MS Core
(615) 343-8601
wade.calcutt@vanderbilt.edu
Small Molecule Consultation
Consultation - Targeted Metabolomics
Consultation - Untargeted Metabolomics
The Proteomics Core offers a comprehensive set of services for analysis of proteins ranging from simple protein identifications and post-translational modification mapping to discovery-based global proteome comparisons and protein-protein interaction studies. State-of-the-art instrumentation, methods, and expertise are available to address almost any need. Our services include but are not limited to:
Kristie L. Rose, Research Assistant Professor
Associate Director Proteomics
(615) 343-1568
kristie.rose@vanderbilt.edu
W. Hayes McDonald, Research Assistant Professor
Associate Director Proteomics
(615) 343-0512
hayes.mcdonald@vanderbilt.edu
Proteomics New Project / Consultation
*if you have additional samples for an existing project please request a sample submission under that project or email the managing director
The Tissue Imaging Core provides direct imaging and profiling of intact tissues by MALDI imaging mass spectrometry. A wide variety of analytes including: proteins, peptides, lipids, endogenous metabolites, and exogenous species such as pharmaceutical compounds or toxins can be imaged in most tissues. Both fresh frozen or FFPE tissues from various sources (human, animal, clinical, plant) are amenable for imaging mass spectrometry. Core personnel assist with experimental design and carry out sample preparation, including tissue sectioning, data acquisition, and data analysis. If you would like to schedule a consultation for imaging mass spectrometry analysis, please click on the button below, and someone will get back to you to discuss your experimental needs.
Michelle L. Reyzer, Research Assistant Professor
Associate Director Tissue Imaging
(615) 343-8371
m.reyzer@vanderbilt.edu
Tissue Imaging New Project / Consultation
Kevin L. Schey, Professor and Director
Director of MSRC Cores
(615) 936-6861
k.schey@vanderbilt.edu
M. Wade Calcutt, Research Assistant Professor
Associate Director of MS Core
(615) 343-8601
wade.calcutt@vanderbilt.edu
Kristie L. Rose, Research Assistant Professor
Associate Director Proteomics
(615) 343-1568
kristie.rose@vanderbilt.edu
W. Hayes McDonald, Research Assistant Professor
Associate Director Proteomics
(615) 343-0512
hayes.mcdonald@vanderbilt.edu
Michelle L. Reyzer, Research Assistant Professor
Associate Director Tissue Imaging
(615) 343-8371
m.reyzer@vanderbilt.edu
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Sergei Chetyrkin |
Staff Scientist |
(615) 343-8433 |
9126 MRB III |
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Brian Hachey |
Research Assistant II |
(615) 343-8433 |
9126 MRB III |
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Mike Leser |
Postdoctoral Fellow |
(615) 343-2700 |
micheal.leser@vanderbilt.edu |
9114C MRB III |
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Purvi Patel |
Research Specialist Sr. |
(615) 343-2700 |
purvi.patel@vanderbilt.edu |
9114C MRB III |
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Audra Judd |
Research Specialist, Sr. |
(615) 936-7935 |
V9160 MRB III |
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David M. Anderson |
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(615) 343-2712 |
V9160 MRB III |
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Hours Open |
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Open Access 8 am to 5 pm Restricted Access 5 pm to 8 am |
8:00 am to 5:00 pm |
465 21st Avenue S 9126 MRB III Nashville TN, 37240 |
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Hours Open |
Hours Staffed |
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Mon – Fri 8 am to 5 pm |
Mon – Fri 8 am to 5 pm |
465 21st Avenue S 9110 MRB III Nashville TN, 37240 |
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Hours Open |
Hours Staffed |
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Mon – Fri 8 am to 5 pm |
Mon – Fri 8 am to 5 pm |
465 21st Avenue S 9160 MRB III Nashville TN, 37240 |
Small Molecule / Metabolomics Core Website
| Name | Role | Phone | Location | |
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| Amanda Renick-Beech |
MSRC Cores Administrative Officer
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amanda.renick@Vanderbilt.Edu
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9160 MRB III
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